Control:Article Title: Aberrant multicellular interferon signaling underlies Adar1 mutation-driven Aicardi-Goutières syndrome-like encephalopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-EIF2AK2 polyclonal antibody Proteintech Cat# 18244-1-AP, RRID:AB_2246451 Mouse anti-GAPDH monoclonal antibody MBL International Cat# M171-3, RRID:AB_10597731 Brilliant Violet 421 anti-mouse CD34 antibody (clone SA376A4) BioLegend Cat# 152207, RRID:AB_2650765 PE/Cyanine7 anti-mouse CD117 (c-kit) antibody (clone 2B8) BioLegend Cat# 105813, RRID:AB_313222 APC anti-mouse CD135 antibody (clone A2F10) BioLegend Cat# 135309, RRID:AB_1953264 PE anti-mouse Ly-6A/E (Sca-1) (clone D7) BioLegend Cat# 108107, RRID:AB_313344 FITC anti-mouse Lineage Cocktail with Isotype Control antibody (clone 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70) BioLegend Cat# 133301 Chemicals, peptides, and recombinant proteins illustra ExoProStar GE Healthcare Cat# US78211 TRIzolTM Reagent Thermo Fisher Scientific Cat# 15596018 Dulbecco’s Phosphate Buffered Saline Nacalai Tesque Cat# 14249-24 BLZ945 Selleck Biotechnology Cat# S7725 Critical commercial assays VeriKine-HS Interferon ALPHA All Subtype ELISA Kit, Mouse PBL Assay Science Cat# 42115-1 Deposited data Bulk RNA-seq data This study DDBJ: DRR893684-DRR893701, DRR893706- DRR893720 Spatial transcriptomic data This study DDBJ: DRR893702-DRR893705, DRR900429- DRR900430, E-GEAD-1189, E-GEAD-1207 Experimental models: Organisms/strains Mouse: Ifih1− /− Oriental Bio Service Cat# OBS:23, RRID:IMSR_OBS:23 Mouse: Ifnar− /− Gift from K. Takeda N/A Mouse: Zbp1− /− NIBIOHN Cat# nbio155 Mouse: Eif2ak2− /− This study N/A Mouse: Tau-cre KI Kumamoto University CARD ID: 1183 Mouse: Gfap-cre Tg Gift from T. Takehara RRID:IMSR_JAX:004600 Mouse: Adar1flox/flox Gift from K. Nishikura RRID:MMRRC_034619-JAX Mouse: Adar1K948N/K948N Inoue et al.44 N/A Mouse: Adar1W197A/W197A Nakahama et al.46 N/A Oligonucleotides Primer for genotyping (Ifih1− /− mice): Primer1 5′-CTCCAACCAACCTGACAGCT-3′ Primer2 5′-TATGCTATACGAAGTTATCTAGAGTCG-3′ Primer3 5′-TTCGATTTCCTTTCCTCCCTATT-3′ This study N/A Primer for genotyping (Ifnar− /− mice): Primer1 5′-CGAGGCGAAGTGGTTAAAAG-3′ Primer2 5′-ACGGATCAACCTCATTCCAC-3′ Primer3 5′-AATTCGCCAATGACAAGACG-3′ This study N/A (Continued on next page) 16 Cell Reports 45, 117113, April 28, 2026 ..
Recombinant:Article Title: Aberrant multicellular interferon signaling underlies Adar1 mutation-driven Aicardi-Goutières syndrome-like encephalopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-EIF2AK2 polyclonal antibody Proteintech Cat# 18244-1-AP, RRID:AB_2246451 Mouse anti-GAPDH monoclonal antibody MBL International Cat# M171-3, RRID:AB_10597731 Brilliant Violet 421 anti-mouse CD34 antibody (clone SA376A4) BioLegend Cat# 152207, RRID:AB_2650765 PE/Cyanine7 anti-mouse CD117 (c-kit) antibody (clone 2B8) BioLegend Cat# 105813, RRID:AB_313222 APC anti-mouse CD135 antibody (clone A2F10) BioLegend Cat# 135309, RRID:AB_1953264 PE anti-mouse Ly-6A/E (Sca-1) (clone D7) BioLegend Cat# 108107, RRID:AB_313344 FITC anti-mouse Lineage Cocktail with Isotype Control antibody (clone 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70) BioLegend Cat# 133301 Chemicals, peptides, and recombinant proteins illustra ExoProStar GE Healthcare Cat# US78211 TRIzolTM Reagent Thermo Fisher Scientific Cat# 15596018 Dulbecco’s Phosphate Buffered Saline Nacalai Tesque Cat# 14249-24 BLZ945 Selleck Biotechnology Cat# S7725 Critical commercial assays VeriKine-HS Interferon ALPHA All Subtype ELISA Kit, Mouse PBL Assay Science Cat# 42115-1 Deposited data Bulk RNA-seq data This study DDBJ: DRR893684-DRR893701, DRR893706- DRR893720 Spatial transcriptomic data This study DDBJ: DRR893702-DRR893705, DRR900429- DRR900430, E-GEAD-1189, E-GEAD-1207 Experimental models: Organisms/strains Mouse: Ifih1− /− Oriental Bio Service Cat# OBS:23, RRID:IMSR_OBS:23 Mouse: Ifnar− /− Gift from K. Takeda N/A Mouse: Zbp1− /− NIBIOHN Cat# nbio155 Mouse: Eif2ak2− /− This study N/A Mouse: Tau-cre KI Kumamoto University CARD ID: 1183 Mouse: Gfap-cre Tg Gift from T. Takehara RRID:IMSR_JAX:004600 Mouse: Adar1flox/flox Gift from K. Nishikura RRID:MMRRC_034619-JAX Mouse: Adar1K948N/K948N Inoue et al.44 N/A Mouse: Adar1W197A/W197A Nakahama et al.46 N/A Oligonucleotides Primer for genotyping (Ifih1− /− mice): Primer1 5′-CTCCAACCAACCTGACAGCT-3′ Primer2 5′-TATGCTATACGAAGTTATCTAGAGTCG-3′ Primer3 5′-TTCGATTTCCTTTCCTCCCTATT-3′ This study N/A Primer for genotyping (Ifnar− /− mice): Primer1 5′-CGAGGCGAAGTGGTTAAAAG-3′ Primer2 5′-ACGGATCAACCTCATTCCAC-3′ Primer3 5′-AATTCGCCAATGACAAGACG-3′ This study N/A (Continued on next page) 16 Cell Reports 45, 117113, April 28, 2026 ..
Saline:Article Title: Aberrant multicellular interferon signaling underlies Adar1 mutation-driven Aicardi-Goutières syndrome-like encephalopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-EIF2AK2 polyclonal antibody Proteintech Cat# 18244-1-AP, RRID:AB_2246451 Mouse anti-GAPDH monoclonal antibody MBL International Cat# M171-3, RRID:AB_10597731 Brilliant Violet 421 anti-mouse CD34 antibody (clone SA376A4) BioLegend Cat# 152207, RRID:AB_2650765 PE/Cyanine7 anti-mouse CD117 (c-kit) antibody (clone 2B8) BioLegend Cat# 105813, RRID:AB_313222 APC anti-mouse CD135 antibody (clone A2F10) BioLegend Cat# 135309, RRID:AB_1953264 PE anti-mouse Ly-6A/E (Sca-1) (clone D7) BioLegend Cat# 108107, RRID:AB_313344 FITC anti-mouse Lineage Cocktail with Isotype Control antibody (clone 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70) BioLegend Cat# 133301 Chemicals, peptides, and recombinant proteins illustra ExoProStar GE Healthcare Cat# US78211 TRIzolTM Reagent Thermo Fisher Scientific Cat# 15596018 Dulbecco’s Phosphate Buffered Saline Nacalai Tesque Cat# 14249-24 BLZ945 Selleck Biotechnology Cat# S7725 Critical commercial assays VeriKine-HS Interferon ALPHA All Subtype ELISA Kit, Mouse PBL Assay Science Cat# 42115-1 Deposited data Bulk RNA-seq data This study DDBJ: DRR893684-DRR893701, DRR893706- DRR893720 Spatial transcriptomic data This study DDBJ: DRR893702-DRR893705, DRR900429- DRR900430, E-GEAD-1189, E-GEAD-1207 Experimental models: Organisms/strains Mouse: Ifih1− /− Oriental Bio Service Cat# OBS:23, RRID:IMSR_OBS:23 Mouse: Ifnar− /− Gift from K. Takeda N/A Mouse: Zbp1− /− NIBIOHN Cat# nbio155 Mouse: Eif2ak2− /− This study N/A Mouse: Tau-cre KI Kumamoto University CARD ID: 1183 Mouse: Gfap-cre Tg Gift from T. Takehara RRID:IMSR_JAX:004600 Mouse: Adar1flox/flox Gift from K. Nishikura RRID:MMRRC_034619-JAX Mouse: Adar1K948N/K948N Inoue et al.44 N/A Mouse: Adar1W197A/W197A Nakahama et al.46 N/A Oligonucleotides Primer for genotyping (Ifih1− /− mice): Primer1 5′-CTCCAACCAACCTGACAGCT-3′ Primer2 5′-TATGCTATACGAAGTTATCTAGAGTCG-3′ Primer3 5′-TTCGATTTCCTTTCCTCCCTATT-3′ This study N/A Primer for genotyping (Ifnar− /− mice): Primer1 5′-CGAGGCGAAGTGGTTAAAAG-3′ Primer2 5′-ACGGATCAACCTCATTCCAC-3′ Primer3 5′-AATTCGCCAATGACAAGACG-3′ This study N/A (Continued on next page) 16 Cell Reports 45, 117113, April 28, 2026 ..
Enzyme-linked Immunosorbent Assay:Article Title: Aberrant multicellular interferon signaling underlies Adar1 mutation-driven Aicardi-Goutières syndrome-like encephalopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-EIF2AK2 polyclonal antibody Proteintech Cat# 18244-1-AP, RRID:AB_2246451 Mouse anti-GAPDH monoclonal antibody MBL International Cat# M171-3, RRID:AB_10597731 Brilliant Violet 421 anti-mouse CD34 antibody (clone SA376A4) BioLegend Cat# 152207, RRID:AB_2650765 PE/Cyanine7 anti-mouse CD117 (c-kit) antibody (clone 2B8) BioLegend Cat# 105813, RRID:AB_313222 APC anti-mouse CD135 antibody (clone A2F10) BioLegend Cat# 135309, RRID:AB_1953264 PE anti-mouse Ly-6A/E (Sca-1) (clone D7) BioLegend Cat# 108107, RRID:AB_313344 FITC anti-mouse Lineage Cocktail with Isotype Control antibody (clone 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70) BioLegend Cat# 133301 Chemicals, peptides, and recombinant proteins illustra ExoProStar GE Healthcare Cat# US78211 TRIzolTM Reagent Thermo Fisher Scientific Cat# 15596018 Dulbecco’s Phosphate Buffered Saline Nacalai Tesque Cat# 14249-24 BLZ945 Selleck Biotechnology Cat# S7725 Critical commercial assays VeriKine-HS Interferon ALPHA All Subtype ELISA Kit, Mouse PBL Assay Science Cat# 42115-1 Deposited data Bulk RNA-seq data This study DDBJ: DRR893684-DRR893701, DRR893706- DRR893720 Spatial transcriptomic data This study DDBJ: DRR893702-DRR893705, DRR900429- DRR900430, E-GEAD-1189, E-GEAD-1207 Experimental models: Organisms/strains Mouse: Ifih1− /− Oriental Bio Service Cat# OBS:23, RRID:IMSR_OBS:23 Mouse: Ifnar− /− Gift from K. Takeda N/A Mouse: Zbp1− /− NIBIOHN Cat# nbio155 Mouse: Eif2ak2− /− This study N/A Mouse: Tau-cre KI Kumamoto University CARD ID: 1183 Mouse: Gfap-cre Tg Gift from T. Takehara RRID:IMSR_JAX:004600 Mouse: Adar1flox/flox Gift from K. Nishikura RRID:MMRRC_034619-JAX Mouse: Adar1K948N/K948N Inoue et al.44 N/A Mouse: Adar1W197A/W197A Nakahama et al.46 N/A Oligonucleotides Primer for genotyping (Ifih1− /− mice): Primer1 5′-CTCCAACCAACCTGACAGCT-3′ Primer2 5′-TATGCTATACGAAGTTATCTAGAGTCG-3′ Primer3 5′-TTCGATTTCCTTTCCTCCCTATT-3′ This study N/A Primer for genotyping (Ifnar− /− mice): Primer1 5′-CGAGGCGAAGTGGTTAAAAG-3′ Primer2 5′-ACGGATCAACCTCATTCCAC-3′ Primer3 5′-AATTCGCCAATGACAAGACG-3′ This study N/A (Continued on next page) 16 Cell Reports 45, 117113, April 28, 2026 ..
RNA Sequencing:Article Title: Aberrant multicellular interferon signaling underlies Adar1 mutation-driven Aicardi-Goutières syndrome-like encephalopathy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-EIF2AK2 polyclonal antibody Proteintech Cat# 18244-1-AP, RRID:AB_2246451 Mouse anti-GAPDH monoclonal antibody MBL International Cat# M171-3, RRID:AB_10597731 Brilliant Violet 421 anti-mouse CD34 antibody (clone SA376A4) BioLegend Cat# 152207, RRID:AB_2650765 PE/Cyanine7 anti-mouse CD117 (c-kit) antibody (clone 2B8) BioLegend Cat# 105813, RRID:AB_313222 APC anti-mouse CD135 antibody (clone A2F10) BioLegend Cat# 135309, RRID:AB_1953264 PE anti-mouse Ly-6A/E (Sca-1) (clone D7) BioLegend Cat# 108107, RRID:AB_313344 FITC anti-mouse Lineage Cocktail with Isotype Control antibody (clone 145-2C11; RB6-8C5; RA3-6B2; Ter-119; M1/70) BioLegend Cat# 133301 Chemicals, peptides, and recombinant proteins illustra ExoProStar GE Healthcare Cat# US78211 TRIzolTM Reagent Thermo Fisher Scientific Cat# 15596018 Dulbecco’s Phosphate Buffered Saline Nacalai Tesque Cat# 14249-24 BLZ945 Selleck Biotechnology Cat# S7725 Critical commercial assays VeriKine-HS Interferon ALPHA All Subtype ELISA Kit, Mouse PBL Assay Science Cat# 42115-1 Deposited data Bulk RNA-seq data This study DDBJ: DRR893684-DRR893701, DRR893706- DRR893720 Spatial transcriptomic data This study DDBJ: DRR893702-DRR893705, DRR900429- DRR900430, E-GEAD-1189, E-GEAD-1207 Experimental models: Organisms/strains Mouse: Ifih1− /− Oriental Bio Service Cat# OBS:23, RRID:IMSR_OBS:23 Mouse: Ifnar− /− Gift from K. Takeda N/A Mouse: Zbp1− /− NIBIOHN Cat# nbio155 Mouse: Eif2ak2− /− This study N/A Mouse: Tau-cre KI Kumamoto University CARD ID: 1183 Mouse: Gfap-cre Tg Gift from T. Takehara RRID:IMSR_JAX:004600 Mouse: Adar1flox/flox Gift from K. Nishikura RRID:MMRRC_034619-JAX Mouse: Adar1K948N/K948N Inoue et al.44 N/A Mouse: Adar1W197A/W197A Nakahama et al.46 N/A Oligonucleotides Primer for genotyping (Ifih1− /− mice): Primer1 5′-CTCCAACCAACCTGACAGCT-3′ Primer2 5′-TATGCTATACGAAGTTATCTAGAGTCG-3′ Primer3 5′-TTCGATTTCCTTTCCTCCCTATT-3′ This study N/A Primer for genotyping (Ifnar− /− mice): Primer1 5′-CGAGGCGAAGTGGTTAAAAG-3′ Primer2 5′-ACGGATCAACCTCATTCCAC-3′ Primer3 5′-AATTCGCCAATGACAAGACG-3′ This study N/A (Continued on next page) 16 Cell Reports 45, 117113, April 28, 2026 ..
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